biliary epithelial cell line h69  (ATCC)

Journal: iScience

Article Title: Polycyclic aromatic hydrocarbons promote tumorigenesis of gallbladder cancer via aryl hydrocarbon Receptor-HEGBC positive feedback axis

doi: 10.1016/j.isci.2025.112505

Figure Lengend Snippet: HEGBC is associated with the PAHs-AhR signaling pathway in GBC (A) The expression of HEGBC in 110 pairs of GBC tissues and adjacent non-tumor tissues was detected using RT-qPCR. Shown are representative blots from 3 experiments. GAPDH was used as a loading control. (B) The representative HE staining pictures of GBC tumor tissues and adjacent tissues (magnification 40×; scale bar, 50 μm). (C) Kaplan-Meier survival analysis of the correlation between high-or low-HEGBC expression and cumulative survival of the 110 GBC patients. The median expression level of HEGBC was used as the cut-off. p < 0.05 by Log rank test. (D) The expression of HEGBC in human non-tumorigenic biliary epithelial cell line H69 and human GBC cell lines (GBC-SD, OCUG-1, NOZ, EH-GB2, and SGC-996) was detected using RT-qPCR. Data are representative of 3 experiments. (E) KEGG functional enrichment analysis of pathways associated with high expression of HEGBC. (F) The heatmap shows KEGG functional enrichment analysis of differentially expressed genes (DEGs) enriched in the HEGBC-related enriched pathways between HEGBC-OE GBC cells and controls. (G and H) GBC-SD and EH-GB2 cells were transfected with the HEGBC-overexpression (OE-HEGBC) plasmids. The expression levels of HEGBC (E) and AhR (F) were analyzed by RT-qPCR. Data are represented as mean ± SD; statistical significance analyzed is indicated by ∗ p < 0.05, ∗∗ p < 0.01, and ∗∗∗ p < 0.001.

Article Snippet: human biliary epithelial cells line H69 , Procell, Wuhan, China , N/A.

Techniques: Expressing, Quantitative RT-PCR, Control, Staining, Functional Assay, Transfection, Over Expression

Journal: iScience

Article Title: Polycyclic aromatic hydrocarbons promote tumorigenesis of gallbladder cancer via aryl hydrocarbon Receptor-HEGBC positive feedback axis

doi: 10.1016/j.isci.2025.112505

Figure Lengend Snippet: PAHs-AhR signaling pathway is activated in GBC (A) The expression of AhR in human gallbladder epithelial cells (H69) and GBC cell lines (GBC-SD and EH-GB2) was detected using RT-qPCR. (B) The expression of AhR in 110 pairs of GBC tissues and adjacent non-tumor tissues was detected using RT-qPCR. (C) The association between AhR and HEGBC was confirmed by Pearson association evaluation ( n = 110 GBC). (D) The portion expression of AhR in GBC tissues and adjacent non-tumor tissues were examined through IHC assay (magnification 10×; scale bar, 200 μm). (E) AhR-dependent luciferase reporter was used to detect AhR activation in GBC cell lines (GBC-SD and EH-GB2). (F) The subcellular localization of AhR in GBC-SD and EH-GB2 cells was analyzed by immunofluorescence. (G) Targeted metabolomics detection of 15 GBC tissues and 15 gallbladder tissues with simple cholecystitis. Volcanic map of differential metabolites (FDR < 0.05, |log2FC|≥1) is shown. (H) VIP scores of differential metabolites. Data are represented as mean ± SD; statistical significance analyzed is indicated by ∗ p < 0.05, ∗∗ p < 0.01, and ∗∗∗ p < 0.001.

Article Snippet: human biliary epithelial cells line H69 , Procell, Wuhan, China , N/A.

Techniques: Expressing, Quantitative RT-PCR, Luciferase, Activation Assay, Immunofluorescence